The ovarian specimens obtained from the patients with 27 malignant germ cell tumors were analyzed in order to study DNA and form factor using flow cytometry and image analyzer. The malignant germ cell tumors consisted of six dysgerminomas, six
endodermal sinus tumors, seven immature teratomas, three teratomas associated with squamous cell carcinoma, two embryonal carcinomas one mixed germ cell tumor and one malignant struma ovarii. Five normal ovaries used as control group. Various
prognostic
factors such as DNA ploidy, S-Phase and measurements of from were evaluated by flow cytometry and image analyzer. Mitotic index, histological grade, nuclear grade and tumor necrosis were assessed with microscope. These prognostic factors of DNA
ploidy,
S-phase, form factor histological were compared with tumor recurrence and clinical stage in this study.
@ES The results were as follows:
@EN 1. Sixteen(59%) out of 27 were aneuploidy and 11(41%) diploidy. All six dysgerminomas and six endodermal sinus tumors were aneuploidy, while six immature teratomas were diploidy.
2. S-phase fractions of malignant germ cell related significantly with those mitotic index(P=0.0201). S-phase fractions significantly increased in mitotic index gradeⅢ compared with grade I(P<0.01). There was no significant different between
grade
I
and Ⅱ, and between gradeⅡ and Ⅲ. There was no difference between S-phase fractions and the remaining histological variables.
3. The incidence of aneuploidy was higher in the high s_ phase fractions(P=0.0041). However, there was no difference between S-phase fractions and tumor recurrence.
4. The incidence of aneuploidy significantly increased in clinical stageⅢandⅣcompared with stage ⅠandⅡ(P=0.0368). However, the difference between clinical stage histological variables was not significant.
5. The difference between form factor and histological variables, between from and tumor recurrence(P=0.3698), and between from factor and S-phase fractions(r=0.76) could not reach statistical significance.
These results suggest that ploidy can give significant value for routine clinical prognostic prediction, whereas hitologic variables and form factor are poorly suitable for the prognostic evaluation.
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